ProductDescription
FiltersforAC48
TheAC48chamberuses35x41mmfilters.Soldinlotsof100.
| PoreSize | 3µm | 5µm | 8µm |
|---|---|---|---|
| CelluloseNitrate | SCC3 | SCC5 | SCC8 |
| StandardPCTE | PFC3 | PFC5 | PFC8 |
| PCTEw/PVP | PRC3 | PRC5 | PRC8 |
Polycarbonatetrack-etch(PCTE)membranesaretheprimarytypeoffiltersusedinourchambersandinstrumentsystems.Thisisaverythinmembranerangingbetween6-10µminnominalthickness.Thismembranetypeoffersexcellentuniformityinporesizeanddensity,aswellasotherbenefitssuchaslowextractables,lowproteinbinding,andnegligIBLeabsorption.Thesecharacteristicsmakeitideallysuitedforuseinchemotaxisassaysaswellasotherhighpurityscreeningassays.
PVPTreatment–PCTEmembranesareavailablepre-treatedwiththewettingagentpolyvinylpyrrolidone(PVP)whichmakesthesurfacehydrophilic.PVP-treatedmembranesareusedwhenthemediasolutionscannotwetoutthemembranebythemselves.Thisoccursprimarilywhenworkingwithverysmallporesizesorhighviscositymediasolutions.CertaincelltypessuchasneutrophilsdonotadherewelltoPVPtreatedmembranesandtendtodropoffaftermigrating.Whilethisisproblematicwhenquantifyingfiltersonthemembrane,someresearchersusingtheChemoTx®sytemortheMB-series96-wellchambersandquantifyingintheplatefindthistobeanadvantage.Extra-cellularmatrixproteinsdonotadherewelltoPVPtreatedmembraneseither.Ifyourassayrequiressuchcoating,werecommendusingPVP-freemembranes.
FiltersfortheAP48,AC48,AA12andAA10arealsoavailableincellulosenitrate.Thesearemuchthicker(~150µm)fibrousfilters,sometimesreferredtoas“torturouspath”filters.Ratherthancountingcellsthathavemigratedthroughthemembrane,asiscommonlydonewithPCTEmembrane,thesefiltersareclarifiedwithsolventsafterapredeterminedincubation,andahigh-resolutionlightmicroscopeisusedtoobservehowfarintothefilterthecellshavemigrated.Thisisalsoknownasa“leADIngedge”study.Thesefiltershavearecommendedshelflifeofoneyear.Theyarecuttoorder,sopleaseallow2-3daysforprocessing.
Polycarbonatemembranesareavailableinthefollowingporesizesandcorrespondingporedensities:
| Poresize(+0/-20%) | 2µm | 3µm | 5µm | 8µm | 10µm | 12µm | 14µm |
|---|---|---|---|---|---|---|---|
| Density(pores/mm2) (tolerance+/-15%) | 2x104 | 2x104 | 4x103 | 1x103 | 1x103 | 1x103 | 5x102 |
Manyfactorsotherthancelltypeenterintotheporesizeselectionprocess.Pleaseseeourpageon ExperimentDesign.
AdditionalInformation
| Weight | 0.1875lbs |
|---|---|
| Dimensions | 5x4x1in |
| PoreSize | 3µm,5µm,8µm |
| FilterMembranes | StandardPCTE,PCTEwithPVP,CelluloseNitrate |
